What method can be used to measure glucosinolates without using chromatography?

05 March 2014 1 2K Report

Biofumigation is the hydrolysis of glucosinolates in brassica species. It's effect is to control fungi and weeds.

It depends greatly on your sample. Tipically, a hydrolysis (either enzymatically of chemically) would yield glucose, this glucose is easily measurable by colorimetric assays (i.e. https://www.caymanchem.com/app/template/Product.vm/catalog/10009582). Knowing the concentration of glucose in the sample, the determination of the concentration of glucosinolates should be straightforward.

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