Why don't you tell us what your codes stand for? The general method for sulfate-reducing bacteria consists essentially in making enrichment cultures and then preparing deep agar (‘ shake ’) cultures or plates in various dilutions. A solid medium containing sulfate, lactate and a trace of ferrous salt is used; the sulfate-reducers form black colonies which can readily be picked off and placed in liquid medium. Inclusion of 3% Na2SO3.7H2O in media eliminates most interfering or contaminating organisms in mixed cultures and greatly facilitates subsequent purification of the sulfate-reducers. This is the Starkey medium: K2HPO4, 0.5 g.; NH4Cl, 1 g.; Na2SO3, 1 g.; CaC12,. 2H2O, 0.1 g. ; MgSO4,. 7H2O, 2 g. ; sodium lactate (70 % solution), 5 g. ; FeSO4, . (NH4)2SO4 . 6H2O, 0.5 g. ; distilled water, 1L (Starkey used tap water) ; pH 7.0-7.5.
SOB-Sulfur oxidizing bacteria could be isolated and cultured in Cha medium or the thiosulfate mineral salts medium: The medium composition in 1L of distilled water is 2.0 g KNO3, 1.0 g NH4Cl, 2.0 g KH2PO4, 2.0 g NaHCO3, 0.8 g MgSO4.7H2O, 5.0 g Na2S2O3.5H2O and 1.0 ml trace element solution with the pH adjusted to 6 with 1N KOH.
For biofilms: See the attached:
Perhaps you might want to go through this article and adapt their methods https://www.nature.com/articles/s41598-018-26342-3
Hi, you may have a look at the EFC Working Party 10 website https://efcweb.org/WP10.html, there you find a guide with some helpful informations, also for the extraction of samples: https://efcweb.org/WP10/_/MICbook.pdf
Dear Okoh,
The less stable corrosion scales tended to be more stable with sulfate increase, which was evidenced by the gradually decreased iron release and the increased stable iron oxides. Bacterial community analysis indicated that when switching to high sulfate water, iron reducing bacteria (IRB) maintained dominant for pipes with stable corrosion scales, while significant increase of sulfur oxidizing bacteria (SOB), sulfate reducing bacteria (SRB) and iron oxidizing bacteria (IOB) was observed for pipes with less stable corrosion scales.
The pipes historically transporting treated surface water can be more stable corrosion scales and only slight increase of iron release occurred when changing to water with multiply higher sulfate; while the pipes historically transporting treated groundwater or blends can be more severe iron release when feed water sulfate concentration increased.
Hope this is helpful for you.
Ashish
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