What may be the reason if I am getting higher mass (calculated mass 420.60 & experimentally 447) in mass spectrometry?
Hello Siju,
We will need some more info to be able to help you with your problem. What is the compound for example, what eluens/buffer you use.
You probably will be measuring an adduct. I will send you a file with the most common adducts and some more info. Hoping it will help you.
File: TOF -LCMS Common_background_contaminant_ions_and_adducts.xls
Kind regards,
Gerrit
Hi,
can you give us some more details (ionisation mode, solvent additives, etc.).
Hello Siju,
We will need some more info to be able to help you with your problem. What is the compound for example, what eluens/buffer you use.
You probably will be measuring an adduct. I will send you a file with the most common adducts and some more info. Hoping it will help you.
File: TOF -LCMS Common_background_contaminant_ions_and_adducts.xls
Kind regards,
Gerrit
Hello
The mass found experimentally should give an indication of the molecular formula. You may have to check if it can be matched by adjusting the mol.wt. Others have suggested that it maybe an adduct but the mass is not close enough to conclude on that. Please check if the calculated value is adjustable by adding two additional carbon for example (420+24). Are you sure of the compound formed?
Please check
Dr.S.Radhakrishnan
You need to look at the ionization mode. It is not clear what ionization mode you have used. Especially in Chemical Ionization using reagent gases like Isobutane, Ammonia etc, you may find adduct ions with higher molecular weight than the actual molecular ion.
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