Greetings,

While PC-12 cells are commonly differentiated using nerve growth factor (NGF), I am going to be using PC-12 Adh (the adherent version of PC-12 cells) for a series of experiments involving NGF.

Here are my questions in case you have used NGF on PC-12 Adh cells:

- What media/supplements did you use for maintenance as well as when differentiating the cells?

- Did you find that the cells grew better in certain plates/flasks?

- What markers did you use to confirm differentiation? Neurite growth/length, gene expression, protein levels, etc.?

Thank you for your help!

Shahin

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