I prepare MSRV semi solid agar and it is melting not frozen even after cooling to normal room temperature.
Road blocked also...... I am baffled by the ZTC term. In this case it would be ZTC =1 and the course mode = 0 (traditional). So why is the odds ratio higher than the odds ratio for ZTC*hybrid?...
23 June 2022 5,858 9 View
Hello there! I need help with this interpretation and would like to ensure I am speaking of this correctly. I am looking at odds a student is successful in a course. The study variable of...
15 June 2022 2,796 0 View
Please find detail: https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/blood-and-cell-culture-dna-kits/?catno=13343 The...
28 March 2022 1,507 0 View
I don't quite understand what exactly they did. How do you actually add a random error component to predicted household income? The Impact of Remittances on Poverty and Human Capital:...
10 November 2020 4,505 2 View
Can anyone show me how the squared poverty gap index can be rewritten as H/n*[u2+(1-u2)CV2)] as in the picture. Step by step please. I have been struggling trying to figure out how to decompose...
07 September 2020 7,072 8 View
I'm conducting a study for my PhD thesis. I've got some constructs from the literature, but the items to measure the constructs were modified (some were added and some were removed). In this case,...
08 June 2020 6,165 9 View
I have a few polyurea samples and I need to determine the crosslink density. Unfortunately the samples arrived with a lot of air bubbles thus mechanical analysis and swelling is not an option. The...
30 October 2019 3,718 4 View
I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...
11 September 2019 9,670 2 View
I'm looking for a pET28 vector with cleavable N-terminal His tag. I noticed there are these two variants (pET28-a and pET-28b). May I know what the difference is between these two?
09 September 2019 7,330 6 View
I remember my mentor saying before that she decided to refrigerate her gentamycin stock solution instead of freezing it since that was the recommended storage condition. Now, I'm dealing with...
04 August 2019 5,991 4 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...
12 July 2024 3,756 4 View
In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?
10 July 2024 6,352 0 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...
02 July 2024 4,887 0 View