I prepare MSRV semi solid agar and it is melting not frozen even after cooling to normal room temperature.
Can anyone show me how the squared poverty gap index can be rewritten as H/n*[u2+(1-u2)CV2)] as in the picture. Step by step please. I have been struggling trying to figure out how to decompose...
07 September 2020 6,867 8 View
I'm conducting a study for my PhD thesis. I've got some constructs from the literature, but the items to measure the constructs were modified (some were added and some were removed). In this case,...
08 June 2020 5,917 9 View
I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...
11 September 2019 9,441 2 View
I remember my mentor saying before that she decided to refrigerate her gentamycin stock solution instead of freezing it since that was the recommended storage condition. Now, I'm dealing with...
04 August 2019 5,766 4 View
To cure my Streptomyces strain of a plasmid, I used 5-FC as a negative selection marker. So, cells without the plasmid (which are the ones I'm looking for) will be able to grow on agar plates...
17 July 2019 5,854 2 View
I am currently using the CRISPR base editing system in bacteria. Unfortunately, I'm not getting any clones with edited bases (not even off-target) but i'm not sure why. I'm quite sure the plasmid...
29 May 2019 8,845 5 View
I've extracted my gDNA from Streptomyces today, on a Friday but it's pretty late and I can't stay late today. Is it okay for me to keep my extracted gDNA over the weekends at -20 degrees Celsius...
11 April 2019 3,139 9 View
I've just done ligation for my plasmid and my next step would be to perform heatshock transformation on Top10 E. coli cells. Some of my colleagues recommended me to use 2 uL to transform my cells...
01 January 2019 10,003 3 View
I have PCR-ed some DNA samples which I need to perform gel extraction on. These DNA samples will subsequently be used for restriction digestion and ligation over the next week. I was advised to...
18 December 2018 4,067 7 View
I've been trying to clone a ~100 bp insert into a ~12000 bp vector backbone. Basically, what I've done is to do the standard things (restriction digestion, PCR cleanup/gel extraction, ligation,...
17 December 2018 959 12 View
The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...
03 March 2021 5,857 3 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...
27 February 2021 8,128 1 View
Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...
26 February 2021 6,253 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...
23 February 2021 2,390 3 View
Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...
22 February 2021 7,980 3 View