What kit/laboratory test should I use for Antithrombin assay based on thrombin inhibition for rat plasma samples?
Am J Pathol. 1988 Feb;130(2):418-26.
Recombinant alpha 1-antitrypsin Pittsburgh attenuates experimental gram-negative septicemia.
Colman RW1, Flores DN, De La Cadena RA, Scott CF, Cousens L, Barr PJ, Hoffman IB, Kueppers F, Fisher D, Idell S, et al.
Helena Laboratories used to have a chromogenic assay kit based on the thrombin substrate S-2238, it works in rat plasma. I checked their website and nowadays Xa is been used instead of thrombin. Contact them to find out if they still have the option since you will need purified standardized thrombin (human or bovine). Biopharm sales purifed thrombin as well as hematological biothecnologies. The attached document has the reference of the assay but it was published in the Proceedings of the New York Academy of Sciences.
Helena Laboratories
800.231.5663
Factor 10a based AT-3 determinations are not disturbed by antithrombin-1 (AT-1 = Fibrin).
Thromb Res. 1977 Oct;11(4):549-53.
Antithrombin (heparin cofactor) assay with "new" chromogenic substrates (S-2238 and Chromozym TH).
Abildgaard U, Lie M, Odegård OR.
Minimal heparin cofactor activity in disseminated intravascular coagulation and cirrhosis. American Journal of Clinical Pathology (Impact Factor: 3.01). 10/1976; 66(3):488-94.
Thrombosis Research Volume 6, Issue 4, April 1975, Pages 287–294