Hello

I have done DNA mutation analysis by NGS using Ion Torrent PGM sequencing instrument. I did amplification of hotspot cancer mutations by using two available panels:

1- Ion Ampliseq colon and lung cancer panel, can detect up to 22 hotspot regions of genes

2- Ion Ampliseq cancer hotspot panel v2, up to 50 genes can be studied.

If you have more questions, I'm happy to tell what I know.

-Omar Youssef

Thank you.

I used the Illumina Miseq to sequence the V3-V4 region of the 16S rRNA gene.

Best

Great! thanks

I used the Illumina Miseq to sequence the V3-V4 region of the 16S rRNA gene to study metagenomics, sometimes i use ion torrent...

Omar Youssef, 

I want to study DNA mutations by NGS using Ion Torrent PGM sequencing instrument  as well. I'm also using CHP v2. May you share your experience,

How much coverage is suitable for detecting SNPs, indels? 

Thank you.