I had a peptide, I am trying to get raman spectra of peptide, I found in literature, the shift in peak could be observed. What kind of shift could be observed red/blue and Why? I had done some experimentation and tried raman spectra of this peptide, however, I am not sure what are my results, most of them are blue shift.
What could be other methods of detection of peptide adsorbed on some surface (TEM, Raman), any other suggestion?
Raman shift means in fact the difference between the wavenumbers (1/lambda, in cm-1) between the incident and scattered light. This difference also corresponds to a vibrational wavenumber (or more exactly the wavenumber corresponding to the transition between two successive vibrational states). So, each Raman peak reveals one of the peptide vibrational wavenumber. Another fact is that Raman peaks are sensitive to the peptide environment, and they can be red- or blue shifted by the interactions. They can also be shifted by conformational transitions of the peptide. You can find several examples of short peptides Raman spectra in our list of references on ResearchGate.
Thankyou for your reply, how about if protein is adsorbed on any surface.
Mr. Tyagi,
In which context there is used the "shift" in the Raman spectra? Due to coordination of the peptide to the metal ions of the surface, for example, the shifting follows the common rule valid for vibrational frequencies, generally.
Dear Ivonova,
The shift in Raman spectra was observed when the peptide adsorbed on surface, however, without peptide their is unique peak was observed only belong to surface.
Mr. Tyagi,
There is possible to have solvent-surface interactions. It would be informative to conduct quantum chemical computations of the Raman and Fs spectra of your surface and surface-solution interacting systems.
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