Is the reaction limited to cell walls or does the oxidation continue for endogenous compounds?
Ammonium persulphate was used for oxidizing and killing bacterial cells and we still used solution of persulphate of ammonium to clean bacterial fixed slides.
In metal oxides how oxygen affect the electrical and magnetic properties? Also, how it impacts the EMI shielding performance. Please make discussion.
05 June 2024 483 0 View
I want to make reconfigurable RF and microwave filter, I need to connect low capacitance (minmum-0.048pF) varactor diode (MA46H120 Series). Can we use this varactor diode (MA46H120 Series) at 6...
21 May 2024 7,504 2 View
We have elastic scattering excitation function data in tabular form and want to obtain partial wave scattering phase shift data for each partial wave say l=0, 1, 2....whats the process to do so...
19 April 2024 2,225 1 View
I have used the DLS machine (Zetasizer Software) to measure the size of micelle prepared. For the first 2 runs the quality report shows "Good", while the 3rd measurement shows as "Too...
17 April 2024 4,491 3 View
I am working on identification of copy number in a recombinant Chinese hamster cell line by southern hybridization. I am having issues with the transfer of DNA onto positively charged nylon...
08 February 2024 2,647 8 View
We did reaction of alpha-bromo-gamma-butyrolactone with aq. ammonia solution at RT to get alpha-amino-gamma-butyrolactone. Reaction completed on TLC. Water distilled completely but unable to...
07 February 2024 5,766 0 View
I tried different protein assay kits (BSA, fluorometric peptide), but it didn't work.
25 January 2024 3,341 0 View
For example, Data obtained from a rheometer is as follows 1. Static yield stress - 1400 Pa, 2. Dynamic yield stress- 640 Pa, 3. Plastic viscosity-68 Pa-Sec, How are these values exactly...
24 January 2024 6,120 0 View
while working with low concentrated mammalian genomic DNA, can we increase the restriction digestion reaction volume to 200 ul to accommodate the required amount of mammalian genomic DNA for...
22 January 2024 6,952 12 View
Dear Researcher Happy New Year-2024. The world is facing different types of health issues today, among all Malaria is one of them. from hundreds of years scientist are working for the controller...
31 December 2023 1,619 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View