What kind of grids are used for TEM test of graphene oxide?

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Which works better for the detachment of HUVECs and HLF from the culture plate? 'Accutase' or 'Trypsin-EDTA' solution?

I am culturing 'Human Umbilical Vein Endothelial Cells' & 'Normal Human Lung Fibroblasts' from the PELOBiotech in their 'Endothelial Cell Growth Medium' & 'Fibroblast Growth Medium'. I am...

16 November 2020 4,671 6 View

Will a recombinant NDV expressing the spike protein of covid-19 be effective?

I know it has to go into trials but it may be safer than other vaccine?

16 April 2020 3,471 3 View

Does anybody have experience with triple or quadruple digestion using Thermo's FastDigest or NEB's HF restriction enzymes?

My double digested plasmid and insert are almost the same size and it is impossible to cut the insert from the gel as it falls behind the plasmid even on a 2% gel. I have been thinking of using a...

14 September 2019 1,044 2 View

Is there any constitutive equation for calculating temperature in friction stir spot welding(FSSW)?

there is an equation that has been developed with abregast et al for calculating temperature in FSW and FSP processes that travel speed of process is also kept in calculation. As the FSSW process...

02 May 2017 2,880 2 View

Can initial grain size affect recrystallization behavior during friction stir welding of austenitic stainless steel?

assume that there is two initial microstructure with the same chemical composition (austenitic stainless steel) the first one has an average grain size of 4 micron and the second one's is about 50...

07 April 2017 2,443 2 View

Is there any constitutive equations for prediction texture in friction stir welding of austenitic steels?

there are several complex equations on predicting texture in friction stir welding of autenitic steels achieved by crystal plasticity modeling but those equations are too complex. Is there any...

26 March 2017 6,482 2 View

Which of three fracture mechanics is better for a specimen with an intentional crack?

there are three different fracture mechanic known as mode 1(tensile opening), mode 2(in-plane shear) and mode 3(anti-plane shear). which one has less detrimental effect on the strength and...

03 March 2017 8,256 4 View

How much is the penetration depth of x-ray beam in a bulk of steel?

does it diffract from near surface, i have different phases in the bulk according to different heat input in welding. can i detect phases by XRD?

21 October 2016 8,604 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Smart grid ideas?

How to use and this is economical?

06 August 2024 3,160 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View