Clone your proof-reading Taq DNA polymerase (Phusion polymerase) PCR products directly into vectors like pJET1.2 (company ThermoFisher Scientific) as this polymerase will give you blunt PCR products and cloning is very easy.

Its very hard to recommend any specific cloning kit. Cloning can vary a lot with the cloning gene and gene origin issues. Theoretically all compatible cloning vectors or cloning kits should work for specific organisms they are designed for, but in practice its not true. Some kits perform good, some not, some even does not work at all. So, I suggest you to search literature for similar kind of studies and try to replicate in your case.

Kamal Kumar I have used PCR master mix (2x) for PCR reaction, the polymerase in this Master mix has the ability to create 3' A overhangs. I have sequenced my PCR product and got NNNNNN seq. at 5' terminals of both primers' product.

1- I purified my PCR product.

2- Get sequenced from seq. company.

3- got results which indicated that the sequence obtained from forward primer has sequence NNNNNN for some nucleotide (aprrox 5-6 nts) from the 5' terminal and sequence from reverse also has NNNNNNN for some nts (5-6 nts) from 5' terminal.

4- when I aligned both sequences and generated a contig, I got complete CDS seq with all correct nts as present in ref sequence.

5- Then I aligned this contig seq. with my reference gene for comparison and I also aligned the proteins sequence of both contig and reference protein. I got same nucleotide and amino acid seq. with equal length and same 3D structure prediction.

I suppose that the presence of NNNNNNN nucleotides from frwd & revrs primer seqs. is the mistake of sequencing machine and it might be possible that complete seq of amplified gene must be present in my PCR product.

Now my question is that is it ok to go for T/A cloning of PCR product?