What kind of characterization assays can I perform to deal with the function of chimeric fusion protein on peripheral nerve regeneration?

More Raju Tippana's questions See All

Are you interested in contributing a book chapter on biomaterials in Orthopaedics?

I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...

29 July 2024 5,015 9 View

Are all indexing bodies for the journal's evaluation similar?

A Guide to Journal Indexing Academic journals undergo evaluation by various indexing bodies to ensure ethical practices and high publishing standards. Three prominent indexes include: · PubMed...

27 June 2024 4,415 0 View

What are applications of Class 1, 2, 3 and 4 steel s/c? Can Class 4 s/c considered as flexural members? Why are Class 4 sections not adopted as beams?

Where are Class 1, 2, 3 and 4 sections generally adopted by steel construction industy? Why are class 4 sections not used as beams? As what structural members are Class 4 members used?

10 June 2024 2,453 1 View

Do you know that the Scopus has released the Cite Score of 2023 today?

Please check these related to your journals at: https://www.scopus.com/sources.uri?zone=TopNavBar&origin=searchbasic The Journal Impact Factor will be released by Clarivate in about two...

04 June 2024 1,281 1 View

How can I calculate the persistence length of a polymer using the gmx polystat command?

Hello everyone. I want to calculate the persistence length of a polymer using the gmx polystat command. It gives the value of the radius of gyration and end-to-end distance in nm. However, for...

04 April 2024 5,346 0 View

Neutralization buffer alternate ?

Typical neutralization buffer used for Protein A/G affinity separations is 1 M Tris/HCl pH 9.0 Any alternate buffer for 1 M Tris/HCl ?

04 March 2024 518 2 View

Is it possible to develop an ML algorithm for NTC, that works at synchronization of line speed (below 30 ms) using FPGA?

Is it possible to develop an ML algorithm for NTC, that works at the synchronization of line speed (below 30 ms) using an FPGA board? Like a next-generation firewall.

26 February 2024 6,254 0 View

Suggest me any good journal papers for the efficient NTC on real time traffic using FPGA and ML algorithms?

I am looking for a few good journal papers for my next work, which is used for efficient network traffic classification (NTC) using FPGA and ML algorithms for real-time NTC. It is a kind of...

26 February 2024 4,037 2 View

Do you know that the hand grip strength is a valuable bio health marker?

The HGS can be measured reliably with a hand-held dynamometer. The cut-off values are different in various populations. It is lower in Asians, women, less educated and privileged, and those...

09 January 2024 4,886 4 View

How can we improve the wettability of Carbon fiber with YSZ slurry?

As there is a significant difference in their CTE, can we make composite out of carbon fiber and YSZ matrix through slurry impregnation..? Even though am desizing the carbon fiber at 550C and...

19 October 2023 330 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Ghanim Al-Khaledi

Hi Raju, Since you are experimenting with a "novel" protein the basics are always applausable, in addition to the cell migration and proliferation assays, Luciferase reporter assay is an addition pls Click link below, if I may suggest also a valid approach will be biochemical profiling. pls see http://www.ncbi.nlm.nih.gov/pubmed/?term=22393241

and for review pls see http://www.stevelacroix.crchuq.ca/documentation/articles/Barrette_et_al_BBI_2010/Barrette_et_al_BBI_2010.pdf

best wishes to you with your research,

http://www.promega.com/~/media/files/resources/cell%20notes/cn021/luciferase%20reporter%20assays-%20powerful%20adaptable%20tools%20for%20cell%20biology%20research.pdf?la=en

Raju Tippana

Hi Ghanim,

Thank you very much

Raju