I'm doing a survey about the optimum method to preservation of bacteria in Microbiology Lab. for my project " Preservation of bacteria "
Dear Ebtehal,
This is some reports about different methods for bacteria preservation, i hope it will help you in this issue.
Kind regards,
Hello,
Freeze-drying, also known as lyophilization used for long term storage of bacteria (biological material) which involves removal of water, freezing the sample, then drying it under a vaccum. Freeze drying method is more advantageous over other methods because its an easy method, application of very low temperature, stability of samples which mainly suitable for many years of preservation.
Dear Ebtehal,
This is some reports about different methods for bacteria preservation, i hope it will help you in this issue.
Kind regards,
Thank you for answer to my question and sharing with me your knowledge and articles
The preservation of bacteria by freezing in glycerol broth
Dexter H Howard
Journal of bacteriology 71 (5), 625, 1956
Hollander and Nell (Applied Microbiol., 2, 164, 1954) reported that the presenceof 15 per cent glycerol protected Escherichia coli, Diplococcus pneumoniae, and Treponema paUidum from the damage of freezing and thawing. Furthermore, T. pallidum could be preserved in 15 per cent glycerol at-70 C for 2 months without any detectable loss of virulence. On the basis of their findings, these authors suggest that dilute glyc-erol solutions might be employed in the preservation of other fragile biological materials. The present report indicates thepotential usefulness of this technique for the preservation of a variety of microorgansms. Cultures of the organisms were grown on hu-man blood agar, chocolate agar or Albimi Brucella agar depending on their nutritional require-ments. After 24-hr incubation at 37 C, the cells were harvested in physiological saline, centri-fuged, and resuspended in Albimi Brucella broth
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The Preservation of Bacteria by Drying in Peptone Plugs*
DI Annear
Epidemiology & Infection 54 (4), 487-508, 1956
1. A new method for the preservation of bacteria by drying is described.
2. Marked differences between organisms were found with respect to the relationship between growth phase and recoveries after drying.
3. Recoveries of several strains of neisseriae and vibrios, both immediately after drying and after storage, were considerably higher than any previously recorded for similar species of these genera.
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nih.gov [PDF]
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Culture preservation
Robert L Gherna, CA Reddy
Methods for General and Molecular Microbiology, Third Edition, 1019-1034, 2007
The primary aim of culture preservation is to maintain the organism alive, uncontaminated, and without variation or mutation, that is, to preserve the culture in a condition that is as close as possible to the original isolate. The major disadvantages of the serial-transfer technique are the risks of contamination, transposition of strain numbers or designations (mislabeling), selection of variants or mutants, and possible loss of culture, as well as the required storage space. Commercially available strips impregnated with Bacillus subtilis spores are used to …
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preservation of bacteria
الباحث العلمي
[PDF] microbiologyresearch.org
The preservation of bacteria by drying
L Stamp - Microbiology, 1947 - mic.microbiologyresearch.org
A quantitative study of the survival of vegetative bacteria on drying in various suspending media led to a simple method for preserving bacterial cultures. Bacterial cells are suspended in melted nutrient gelatin cont. aining ascorbic acid or sodium ascorbate in …
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Preservation of bacteria by lyophilization
RJ Heckly - Advances in applied microbiology, 1961 - Elsevier
Publisher Summary This chapter describes the preservation of bacteria by lyophilization. Lyophilization, or freeze-drying, is a term applied to the procedure of freezing and subliming water from frozen preparations.“Lyophil method” is specifically applied to the condensation …
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Chapter II culture collections and the preservation of bacteria
SP Lapage, JE Shelton, TG Mitchell… - Methods in …, 1970 - Elsevier
Publisher Summary This chapter discusses the culture collections and the preservation of bacteria. It discusses a general account of culture collections of bacteria, and an account of the day to day management and operation of a culture collection. Methods of maintenance …
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[HTML] wiley.com
Survivability and long‐term preservation of bacteria in water and in phosphate‐buffered saline
CH Liao, LM Shollenberger - Letters in applied microbiology, 2003 - Wiley Online Library
Aims: To evaluate the suitability of using sterile water and phosphate‐buffered saline (PBS) for preservation of bacteria pathogenic to plants or humans. Methods and Results: The stationary‐phase bacterial cells collected from rich agar media were transferred to 10 ml of …
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عمليات البحث ذات الصلة
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The preservation of bacteria by freezing in glycerol broth
DH Howard - Journal of bacteriology, 1956 - Am Soc Microbiol
Hollander and Nell (Applied Microbiol., 2, 164, 1954) reported that the presenceof 15 per cent glycerol protected Escherichia coli, Diplococcus pneumoniae, and Treponema paUidum from the damage of freezing and thawing. Furthermore, T. pallidum could be …
تم اقتباسها في عدد: 63 مقالات ذات صلة
Culture preservation
RL Gherna, CA Reddy - Methods for General and Molecular …, 2007 - asmscience.org
… Freeze-drying (lyophilization) is one of the most economical and effective methods for long-term preservation of bacteria and other microorganisms. Two of the most common methods used are centrifugal freeze-drying and prefreezing …
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[PDF] nih.gov
The Preservation of Bacteria by Drying in Peptone Plugs*
DI Annear - Epidemiology & Infection, 1956 - cambridge.org
1. A new method for the preservation of bacteria by drying is described. 2. Marked differences between organisms were found with respect to the relationship between growth phase and recoveries after drying. 3. Recoveries of several strains of neisseriae and vibrios …
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Chapter I Media for the maintenance and preservation of bacteria
SP Lapage, JE Shelton, TG Mitchell - Methods in microbiology, 1970 - Elsevier
Publisher Summary This chapter discusses the media for the maintenance and preservation of bacteria derived from the experience of the National Collection of Industrial Bacteria (NCIB), National Collection of Marine Bacteria (NCMB) and the National Collection of Type …
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[PDF] academia.edu
Preservation of bacteria in natural polymers
AA Krumnow, IB Sorokulova, E Olsen, L Globa… - Journal of …, 2009 - Elsevier
A new inexpensive and simple method for preserving microorganisms has been developed. Natural polymers of acacia gum and pullulan were used to preserve model bacteria Escherichia coli and Bacillus subtilis via immobilization and storage under various …
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[PDF] wiley.com
The rate of horizontal transmission of antibiotic resistance plasmids is increased in food preservation‐stressed bacteria
MAS Mc Mahon, IS Blair, JE Moore… - Journal of applied …, 2007 - Wiley Online Library
Aim: The aim of this study was to investigate the possibility that sublethal food preservation stresses (high/low temperature, osmotic and pH stress) can alter the rate of horizontal transmission of antibiotic resistance (ABR) plasmids between Escherichia coli strains and …
Preservation of microorganisms by desiccation can be preferred method for long term storage of cultures for years. There are extensive culture collections that depend on these drying methods to preserve a huge diversity of cells for future action of widely spreading and promoting the theory, .
We know by way of conservation that it is a good method and used by specialists.
Thank you Shonke Molokoane and Adraaa Alfatlawy for your answer
Dear Ebtehal ; use this reference
[Methods for preserving bacterial cultures] [Methods for preserving bacterial cultures]
https://www.ncbi.nlm.nih.gov/pubmed/9629607
Dear Sumaya Aldabbagh Thank you for sharing with me your knowledge and article
Anaam Jawad Alabbasy thank you for your answer.
I ask you does McConkey slant agar use to preservation both type of Gram positive and negative bacteria or just for G -ve bacteria ?
How long storage your isolates on it?
Dear Dr. Sajda Taha Mahmood thank you very much for your answer and sharing this article with me, best regards
Hi,
agar slants for a shorter period of storage and glycerol stocks for a longer period of storage.
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