SPSS - how to create new variable include and excluding cases based on another variable?

Hi there, I have been searching and searching and cannot find an answer to this problem... I have a variable (Ref_Accepted_Comb) for the number of referrals accepted with values of (1=Yes, 2=No,...

08 February 2021 3,479 3 View

Does maternal potentiation work?

Has anybody been able to replicate finding of papers that show increased vocalization in mice pups after a brief period of interaction with their dam, also called maternal potentiation? We have...

11 January 2021 558 2 View

Anybody know how can I analyze differences between groups vocalization syntax using the transition probability between syllables?

I have been using a MATLAB script called DeepSqueak to analyze vocal communication in mice. One of the outcomes of this analysis is the transition probability between the different syllables that...

09 December 2020 1,525 1 View

Anyone with experience conducting proximity ligation assay (PLA) in mouse heart tissue ?

I was wondering if anyone had any experience doing proximity ligation assays (PLA) using mouse whole heart (ventricular) tissue (in any state)?

29 November 2020 9,103 1 View

How to extract/remove ethanol from kava tincture?

Producing Kava tincture using different alcohol concentrstion as solvent, soaked for 3 weeks filter off the kava paste and left with mixture of kavalactones and ethanol..need a method to remove...

14 June 2020 5,104 1 View

Can you have one variable load onto two factors in a CFA, prior to SEM?

I am running a SEM, and I have 6 variables which will load onto two factors (positive and negative mental health). However, this model does not meet a good model fit. When I allow depression to...

19 May 2020 8,286 3 View

When does someone use corrected fin length and the actual fin length in heat transfer problems?

In some instances the extended length of a fin is used but then in other instances the normal fin length is used in extended surface heat transfer problems.

23 March 2020 7,553 4 View

How will I calculate the surface temperature of a HDPE pipe that is exposed to the sun?

I need to calculate the final temperature of a pipe that's been lying in the sun for the day, however, I am unsure how to do this. I know how to calculate the heat transfer through the pipe wall...

19 January 2020 1,259 10 View

Mouse islet isolation without pancreas perfusion?

Is there a current/reliable way to isolate mouse islets (keeping their morphology and viability for culturing in vitro) without perfusing the pancreas through the bile duct?

14 September 2019 3,577 1 View

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19 October 2018 6,019 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

How to find statistical data of EEG signals?

Hi all, I hope everyone is doing good. I am working on Machine Learning, I am working on EEG data for which I have to extract statistical features of the data. Using mne library I have extracted...

29 January 2021 6,686 1 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Is PV array not available in MATLAb 2020a simulink?

I have MATLAB 2020a. PV array is not present in the simulink library. Can anyone please explain if the name of module has been changed or is it not available anymore? Is there any way to add PV...

24 January 2021 7,962 5 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

No title

13 January 2021 2,487 1 View

IET Digital Library Access?

Hello to everyone, I do not have access to the IET Digital Library at the institution where I work. Can those who have access to this library send me the publication whose DOI is given below?...

13 January 2021 9,385 2 View