Does anyone know the typical markers for cytoplasm or nucleus after fractionation? KRT18 for cytoplasm? Histone H3 for nucleus? Also, does anyone know a fractionation protocol for uterine epithelial cells (IKA cells)? Thanks
Lamin A/C is also a good marker for nuclear fraction but sometimes difficult to see by WB because it can remain in the insoluble fraction. Sonication of your nuclear fraction should solubilize lamin A/C.