Direct and Indirect Experimental Measurements to Measure Activation Energy Reduction of Biological Enzymatic Reactions
Carry out the reaction with and without enzyme, then measure and compare the rates of the two reactions. I'll leave the details for you.
Thank you Sir. Please leave me the details when you feel free. My email is : [email protected]
The activation energy is measured by measuring the reaction rate as a function of temperature and making an Arrhenius plot. A previous post on RG covered this nicely:
https://www.researchgate.net/post/What_is_the_protocol_for_finding_activation_energy_using_an_arrhenius_plot
Note that you need to determine two Energies of Activation, Ea: Ea(with enzyme) and Ea(without enzyme), each determined using the procedure posted above. The difference between them is the Ea reduction you are looking for. The first Ea should be lower than the second Ea, if the enzyme is working as expected.
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