What is the standard quantity of mastermix for SSR markers in the chickpea?

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Yordan Muhovski

Hi, check in some publications.

Mohammad Anwar Khan

No common standardization for this, it depends on your lab conditions and objectives. Normally labs use 20micro Mol master mix conc.....this may work for you also.

Nedup Dorji

I used 50 ng/μl, I think it could go even 30 ng/μl.

P J Paul

Thank You so much to all.

Ravinder Kumar

There is no such type of standard quantity of mastermix. you have standardized according to your lab conditions and Thermal cyclar.

Thank you all again for the answers..