Hi Urvashi,

Then, antioxidant determination by DPPH assay there are different ways to express your data. Your data can be expressed in IC50, % inhibition, and umol TE/g, the your choice will depend of literature used to compare your results.

Best regards

Hi Urvashi,

Then, antioxidant determination by DPPH assay there are different ways to express your data. Your data can be expressed in IC50, % inhibition, and umol TE/g, the your choice will depend of literature used to compare your results.

Best regards

Hi Urvashi 

You can follow the attached paper which is perfect for DPPH assay. Herein you will find the formula to calculate the % inhibition along-with details of the method and you can compare your different samples easily by calculating the IC50 values. I hope it should be helpful. If you need further assistance, feel free to ask. Regards 

Different concentration of methanolic extract of plant taken like 10mg/ml,20mg/ml,30mg/ml,40mg/ml,50mg/ml and then added a fixed concentration of DPPH solution to all. then just measure uv absorbency in 517nm after 30min - 60min incubation against a blank.  

You need to understand the mechanism behind the protocol first then you can apply it judiciously to your plant material.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551182/

http://www.thaiscience.info/journals/Article/The%20use%20of%20the%20stable%20free%20radical%20diphenylpicrylhydrazyl%20%28dpph%29%20for%20estimating%20antioxidant%20activity.pdf

Check the following file for several threads on the subject of anti-oxidant activity:

You may see this 

http://www.sciencedirect.com/science/article/pii/S2095177915000131

THANK YOU SO MUCH ALL FOR YOUR VALUABLE SUGGESTIONS ....