Srijan Acharya

3 Questions 4 Answers 0 Followers

Questions related from Srijan Acharya

What does pmol/mg meaning?

Usually receptor expression unit is pmol/mg of protein. What does it mean? I found some article publish that their receptor expression level is 1.5-2 pmol/mg protein. What does it mean? When we...

09 September 2016 1,293 1 View

How can I prevent cell culture contamination?

we usually used HEK293 cell line for analysis our results, but for last few weeks I suffer a lot of cell contamination. Characteristics of contamination is also unique. To prevent contamination I...

06 June 2015 5,895 13 View

How can I improve my HEK293 cell transfection?

I use HEK293 cell lines and trying to transfection D3R. My protocol is 2.5 microgram DNA is added to 100 microliter serum free media, then added 18 microliter frozen polyethylimine (PEI) and...

04 April 2015 6,834 22 View