Hi, I am working on antioxidant property of medicinal plants, extract prepared in hexane and chloroform (non polar solvents). While working with these extracts, i am facing a problem as all the reagents of different tests (phenol, flavonoids, FRAP, DPPH, ABTS, etc) are prepared in water or methanol (polar solvent), and these non-mixable with plant extracts (hexane and chloroform extracts). so when i added extract and reagents together two separate layer formed and absorbance of this mixture also misleads. So Can anyone suggest me any solution to overcome this problem?

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