What is the smallest sized particle which can be studied through FACS?

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What are the percentages of Lymphocytes, B cells and T cells in murine splenocytes?

Hi, What are the percentages of Lymphocytes, B cells, T cells, CD4 cells, CD8 cells, Macrophages, DCs and NK cells in the mice spleen? what is the proportion of T and B cells in lymphocytes of...

31 December 2017 1,397 7 View

Can peristaltic pumping system in cytoflex of BC show some adverse affect on the cell condition?

Hi, Can peristaltic pumping system in cytoflex of BC show some adverse affect on the cell condition? As cells may need to pass through a narrow tube for peristaltic movement by the pump. Thanks

02 March 2016 9,985 1 View

Why some people do live cell gating for Flow Cytometry study, even in the experiment involving intracellular staining?

For intracellular staining (such as for cytokines and transcription factors) in flow cytometry, we need to FIX & Permeablise the cells, prior the staining with fluorochrome-tagged antibodies....

02 March 2016 4,693 4 View

How much it is safe to use generic medicine?

Ethical Medicines are costly, but as and when the patent of a drug expires, any other company can composite the drug and cell it with much lower cost. Due to little variation of composition...

11 December 2015 7,183 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View