Mohammad Asad

5 Questions 33 Answers 0 Followers

Questions related from Mohammad Asad

What is the smallest sized particle which can be studied through FACS?

Hi, I have a liposome of size 200-300 nm. If it will be labelled with fluorescence antibody, whether it can be studied through FACS? It is stable for FACS studies and we have LSR FORTESSA (BD) and...

02 February 2018 9,451 2 View

What are the percentages of Lymphocytes, B cells and T cells in murine splenocytes?

Hi, What are the percentages of Lymphocytes, B cells, T cells, CD4 cells, CD8 cells, Macrophages, DCs and NK cells in the mice spleen? what is the proportion of T and B cells in lymphocytes of...

01 January 2018 1,454 7 View

Why some people do live cell gating for Flow Cytometry study, even in the experiment involving intracellular staining?

For intracellular staining (such as for cytokines and transcription factors) in flow cytometry, we need to FIX & Permeablise the cells, prior the staining with fluorochrome-tagged antibodies....

03 March 2016 4,789 4 View

Can peristaltic pumping system in cytoflex of BC show some adverse affect on the cell condition?

Hi, Can peristaltic pumping system in cytoflex of BC show some adverse affect on the cell condition? As cells may need to pass through a narrow tube for peristaltic movement by the pump. Thanks

03 March 2016 10,120 1 View

How much it is safe to use generic medicine?

Ethical Medicines are costly, but as and when the patent of a drug expires, any other company can composite the drug and cell it with much lower cost. Due to little variation of composition...

12 December 2015 7,247 1 View