There are many complicated and detailed steps, devices like cold centrifuge, O.D measurement, silica gel, columns, HPLC, If possible please i need a short recap of the procedure and advices with Many Thanks to you
Ali
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Faction by ethyl acetate.
Or, add 75-80% methanol, cover it with foil, evaporate slowly.
Dry pack column chromatography with silica beads.
A simple method:
Obtain a pure culture broth of P. aeruginosa.
Centrifuge the broth.
Separate the supernatant.
Add chloroform to the supernatant.
Keep it for settlement.
Add HCl. It will turns into pink/red. Again keep for settlement.
Add NaOH, it will turns blue.
Pigment isolated.
Chloroform can be used for Extraction of pyocyanin. HCl (acid) can be use to decrease pH, turns red. NaOH (alkaline) can be used to increase pH, turns to blue. Addition of acid and alkali is done, to neutralization and purification.
The simplest protocol for the extraction and purification of pyocyanin from Pseudomonas aeruginosa involves the following steps:
It is important to note that this simplified protocol may yield a crude pyocyanin extract, and further purification steps might be required for obtaining a highly pure compound. Additionally, it is advisable to ensure proper safety measures and use sterile techniques throughout the extraction and purification process to avoid contamination and ensure accurate results.
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