hey, i want to estimate flavonoids in wheat leaves grown under saline and high temperature stress. kindly share with me simplest methods and steps involved in calculations.
Regards
Dear Wajid Mahboob,
Please see this link
https://www.researchgate.net/post/Does_Total_Flavonoid_Content_determination_requires_Potassium_Acetate_as_reagents_when_Quercetin_is_used_as_standard_compound
Best regards
Aly
Hi Wajid,
Normally detection of flavonoid is not a problem, so if you have the material to do so, I think you can go for a metabolomic approach using HPLC-UV (or MS even better). Your project sounds very interresting!
Regards,
Paul
Spectrophotometric method using alumunium chloride reagent is one of the simple method. Total flavonoid calculated as rutin equivalent (RE) or quercetin equivalent (QE) depend on the standard used. The main problem of this method is the selevtivity, sometime we found that the value of intercept is great that due to lack of selectivity. So, the HPLC or LCMS is a good alternatives but not simplest method,
Dear Wajid
Find in attachment a simple spectrophotometric method.
For the steps involved in calculations, perform the assay and come back with Abs values.
Regards!
NOBOSSE.
Hey Wajid, a very simple method is the spectrophotometric method described by Chang. I attach the file for you.
Good luck!!
Hello, I think the best method is used the assay spectrophotometer.
you will find enclosed an article that explains the method.
cordially
Article ANTIOXIDANT AND ANTI-INFLAMMATORY PROPERTIES OF ETHANOLIC EX...
The best method is spectrophotometric assay, followed by HPLC analysis.
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction