Do you mean "Reverse Transcriptase PCR" or "Real-time PCR" or "Real-time reverse-transcriptase PCR"?

If you tell us your question and protocol, we can help you with your data. Also, you need to talk with your mentor. You shouldn't be setting up experiment if you don't understand the the protocol.

RT-PCR means you convert the RNA to DNA before you amplify the DNA using PCR.

For PCR, you just amplify DNA based on your template.

Dear Merieme

I agree with Dr. Clark. Provide more context to your question so people from the RG community can provide you with helpful answers. RT-PCR may mean two things: reverse transcriptase PCR or real-time PCR. For a good introduction on these methods:

http://www.primerdesign.co.uk/assets/files/beginners_guide_to_real_time_pcr.pdf

https://www.thermofisher.com/ph/en/home/references/ambion-tech-support/rtpcr-analysis/general-articles/rt--pcr-the-basics.html

All the best,

Arizaldo

Thank you all.

I mean real time PCR.

Hi Merieme...

Its a huge difference, and you have to be more specific! You still have a chance to learn, keep going!

Kind Regards.

Real-time PCR gives a very precise measurement of your template. It's most commonly used to measure gene expression as it's much more sensitive than traditional end-point PCR.

Now, are you using a standard curve or are you looking at relative expression values?

RT-PCR => RNA contre to DNA (RT)=> DNA amplification (PCR)

PCR => DNA amplification

you should better search internet, there are thousands of blogs, articles, papers and videos available. Please do some efforts yourself to find the answers. Lets now end this post.

Dear merieme

Two significant responses are:

First, when we want to maintain for a while an RNA fragment, because the RNA is unstable, we can convert it to CDNA by RT-PCR.

Second, when we want to increase the number of RNA fargment to be better detected in many infections with RNA genome, we use RT-PCR.

The PCR technique of DNA replicates a lot of parts, whereas the RT-PCR technique initially raises the RNA fragment into DNA and then increases its fragmentation .

It is not that difficult question. I suggest to use google or youtube.

RT-PCR or qPCR always applied to evaluate the exact expression levels of different genes in a specific time point.