I have some mutations (Arginine to Proline) in extended-spectrum beta-lactamase (ESBL) in Klebsiella pneumoniae bacteria, what is the expected effect of these mutations on the activity of this enzyme ?
Have you seen the article by Vanhove et al? (Investigation of the folding pathway of the TEM-1 beta lactamase, Proteins (1995) 22:110). I dont know if the same applied to your beta lactamase though.