What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Quantification of GC-MS data, issue with Masshunter, Any ideas?

Hi, I am using the Masshunter software to quantify my GC-MS data. So, did the integration using MassHunter. but the data (area) are weird. I have a lot of peaks in one chromatogram, therefore,...

09 November 2020 2,181 5 View

How to apply cyclic loading to a discrete model of a masonry wall in 3DEC software?

It is possible to apply a monotonic load to a URM wall in 3DEC code but I am wondering is it possible to apply a cyclic load there like FEM softwares?

10 February 2020 3,237 3 View

Can pET expression vectors get used for sequencing and storage?

I was wondering if we could skip one step in cloning process from sequencing vector to expression vector? Meaning that start cloning the gene directly into expression vector such as pET 28b and do...

04 February 2020 9,742 2 View

Do you recommend ligation free cloning system?

I plan to clone some terpene synthase genes into a sequencing vector and later do the heterologous expression using an expression vector. I was wondering if anyone has experience with free...

28 January 2020 7,751 3 View

Why R studio deletes some rows from the heatmap/ doesn't show all genes?

I am using Rstudio to create heatmap for some expression data; My file includes 38 genes, therefore, 38 rows and 20 columns as samples. The output heatmap shows only 19 of my genes!!! Any idea how...

10 December 2019 7,530 3 View

What software/program you recommend to convert fasta file to Nexus.nxs?

I need to convert my fasta format alignment file to "Nexus.nxs" to be able to use it in Mybeys. What is your suggestion? So far the files I created using Mega x as well as an online database...

09 October 2019 4,362 2 View

Problem with trimAL installation?

Is anyone can help in how to install trimAL on windows 10? I am gonna use the software for trimming of my aligned sequences. It seems the software does not get installed in my system! Any idea...

15 September 2019 6,068 3 View

What will be the programming language of future Computational Codes? Good old FORTRAN evolves or will be removed from heavy duty computational world?

I know there are great reliable libraries on Fortran for Computational PDE/ODE, Computational Statistics and Numerical Linear Algebra. Also almost all the legacy software packages of Civil...

30 August 2018 5,424 4 View

Do I need to design a specific antibody for purification of my small recombinant peptides?

I am fusing small peptides around 10 to 30 a.a into the C-terminus of a larger protein. The question is if I need to design specific antibodies against those small epitopes? I actually have two...

10 May 2018 1,091 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View