Dear Wang, if you think that the MIC value is really impressive then you should go first for invivo and check its results invivo after that you can go for its mode of actions.

Dear Jiayi,

I suggest to do the following:

1-Screen the activity of the new antibacterial agent on all available bacteria in order to know on what kind of bacteria this agent is effective.

2-Determine its MIC value and compare it with known antibacterial agents. This is in order to compare its potency to that of other anti-bacterial agents.

3-Conduct In vivo study in order to determine the new anti-bacterial agent's pharmacokinetics and metabolism.

4-Conduct pharmacology experiments to determine the the anti-bacterial mechanism of action.

Generally, if the antibacterial agent is found to be effective against a number of bacteria that known antibacterial agents are effective against, the mechanism of action of the new antibacterial agent is expected to be the same as for those antibacterials agents.

Hoping this will be helpful,

Rafik

In my opinion, the next thing you should do is check the substance for inhibition of growth of mammalian cells in culture and for hemolysis. A substance that kills bacteria but is also toxic to the host is unsuitable for internal use, but can still be useful as a topical agent or in ex vivo applications.

Dear Prof. Rafik:

Thanks for your helpful reply, I have copied these suggestions in my notebook. I want to ask another question, if i want to compare my antibacterial agent with know agents, does it exist a database to search or read the paper?

Dear Prof. Adam B Shapiro:

I previously believe if this agent has toxic in vivo, it might be forgeted. After reading your suggestion, I have found another way, Thanks a lot!

Thanks a lot Dr. Syed Shams ul Hassan.

Dear Jiayi,

You can find in the literature the C50 of many known antibacterial agents. If you send me the kind of bacteria that your agent works against, I can check for you on the known antibacterial agent that fits this data,

Rafik

Dear Jiayi

The main steps are necessary to be followed:

a-Verify the antibacterial activity of selected agent on clinical and reference strains.

b-Investigate the solvent which give better activity of agent

c-Check the MIC of agent

d-Purify and optimize activity of agent

4-Realize the in vivo test and compare with standard pharmaceutical agent

Thank you

Marius