I make scaffold of 6% gelatin of freeze drying method.. i would prefer to know the suitable required time and concentration of glutaraldehyde to make cross-linking to gelatin after freeze drying
Dear Hoda, thank you for posting a very interesting technical question on RG. I'm not a specialist in this field of research enough to give you a qualified answer, However, I can suggest to you the following relevant literature reference which might help you in your analysis:
Synthesis and characterization of glutaraldehyde-based crosslinked gelatin as a local hemostat sponge in surgery: An in vitro study
Article Synthesis and characterization of glutaraldehyde-based cross...
Fortunately this paper is freely available as public full text on RG.