I'm working with a rickettsia symbiont found in some insects, and I found some variability of the bacteria present in different populations of the same species of insect.
I have characterized the bacteria using 16S, gltA and atpA finding a different variability in these 3 regions.
I'd like to know what is the most conserved region in the genome of Rickettsia among the atpA, the glta and the16S. I believe the correct order is what I have just written. atpA should be the most conserved region, gltA should be a little less conserved and 16S should be the region that changes faster. Can anyone suggest me a paper on this topic? Many thanks in advance
We don't know much about atpA, but 17kDa is a highly conserved region that is routinely used.
Dear Lindsay,
many thanks for your suggestion.
After I posted my question I used a highly variable rpm region and I had interesting results.
I will test also 17kDa to check if this region is more conserved than atpA.
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