We will conduct a laboratory procedure that measures the serum nitrite/ nitrate. We do not know what instrument will be used that is easier and less expensive to conduct this research. Thank you!
If HPLC/ion chromatography and mass spectrometry would be the best choices, There are also photometric methods that are technically demanding. This article may give you an idea of possibilities.
Article Comparison of nitrite/nitrate concentration in human plasma ...
Clinical chemistry tests are focused on detecting and in quantifying different samples like blood or urine, the most commonly tested specimens. Sodium nitrite can be measured using an inidivudal's plasma/serum sample (not limited to that). But to think of what would be the better recommendation for detection nitrates in the body is by using blood samples.
In the laboratory, measuring the quantities of plasma and serum frequently uses mass spectrometry. However, according to numerous published studies and journals, the colorimetric approach is more effective for measuring nitrate/nitrite levels. By producing a colored output, this colorimetric approach calculates the susbtance concentration in the specimen which will then give the result for the sodium nitrate. After that, the outcomes are read using a spetrophotometer. The Beer-Lambert law, which asserts that a solute's concentration is proportionate to its absorbance, can be used to support the absorbance component of the colorimetric approach (giving the colored result).
Different links about sodium nitrate/nitrite determination:
Article Colorimetric Method for Determination of Nitrate
http://www.orientjchem.org/vol34no6/spectrophotometric-method-for-determination-of-nitrite-and-nitrate-levels-in-broccoli-and-cauliflower-with-different-fertilization-treatment/#:~:text=Determination%20of%20Nitrite%20and%20Nitrate%20levels%20were%20done%20by%20the,11%20minutes%20to%2018%20minutes.
In terms of automated instruments, if available, you can use Roche’s Cobas Mira.
Tatsch, Etiane & Bochi, Guilherme & Pereira, Renata & Kober, Helena & Agertt, Vanessa & Campos, Marli & Gomes, Patrícia & Duarte, Marta & Moresco, Rafael. (2011). A simple and inexpensive automated technique for measurement of serum nitrite/nitrate. Clinical biochemistry. 44. 348-50. 10.1016/j.clinbiochem.2010.12.011.
According to the study about the automated technique for the measurement of serum nitrite/ nitrate, it is proven that using the Cobas Mira Clinical chemistry analyser applying the modified Griess method is simple and inexpensive but linear, precise and accurate.
Tatsch, E., Bochi, G. V., Pereira, R. da S., Kober, H., Agertt, V. A., de Campos, M. M. A., Gomes, P., Duarte, M. M. M. F., & Moresco, R. N. (2010, December 23). A simple and inexpensive automated technique for measurement of serum nitrite/nitrate. Clinical Biochemistry. Retrieved February 21, 2023, from https://www.sciencedirect.com/science/article/abs/pii/S0009912010005229?via%3Dihub
There may not be many Miras around anymore but if it works on them then it works on any other clinical analyzer.
Nitrates and nitrites are compounds that can be found in different foods and naturally in the human body. These compounds are significant as they are helpful in preventing the growth of bacteria, improving the appearance of meat and for the growth of plants. Nitrite when losing an oxygen molecule, becomes nitric oxygen which is both harmful and helpful for the body. It can be harmful as an excess amount can be toxic. These compounds are significant to the blood as they can help in dilate the blood vessels and lower blood pressure (Gunnar, K., 2020). These compounds can then be measured through different chemical analyzers. According to Tatsch et. al (2010), one of the automated analyzers for serum nitrites and nitrates is the Cobas Mira chemistry analyzer. The chemistry analyzer uses a modification of the Griess Method as it is specific for detecting nitrites. This method for detecting nitrites is simple and inexpensive not needing any other expensive equipment. The result of the chemistry analyzer has shown to be linear, precise and accurate.
References:
Gunnar, K. (2020). Are nitrates and nitrites in foods harmful? Healthline. Retrieved February 23, 2023 from https://www.healthline.com/nutrition/are-nitrates-and-nitrites-harmful
Tatsch, E. et. al (2010). A simple and inexpensive automated technique for measurement of serum nitrite/ nitrate. Clinical Biochemistry, 44 (4) 348-350. Retrieved February 24, 2023 from https://www.sciencedirect.com/science/article/abs/pii/S0009912010005229
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Infectious Diseases