I am performing bi-layer well diffusion antibacterial assay of plant extracts. At the bottom of the plate, I pour hard agar media ( nutrient broth + 1.5% agar). Then, I mix bacteria from broth culture with soft agar media ( nutrient broth + 0.8% agar) and pour it on top. In the soft agar, I make well where I load the sample. But only when using gram-negative bacteria, I get this kind of bubbles (apparently) in the media. There has been no problem with bacterial growth. The same thing happened with the TSB media.
Dear suvroto
Hi
In the microbiology department of the laboratory where I work, when the suspension of different dilutions of food samples containing bacteria (gram-negative) is poured into a plate and then cultured in pour plate method with the "plate count agar" medium, these bubbles are formed. In my opinion, due to the fact that some gram-negative bacteria, especially Enterobacteria, produce gas after being exposed to suitable nutritional conditions for growth and reproduction (this process is used to detect the presence of bacteria such as coliform and E.coli in broth medium) and these gases cannot leave the culture medium after solidification, they appear in the form of bubbles. In any case, the formation of bubbles in the culture medium is a natural issue and does not affect the work process and the results.
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