What is the reason of getting this kind of bubbles in nutrient agar media only when mixing gram negative bacteria in the media and let it solidify?

I am performing bi-layer well diffusion antibacterial assay of plant extracts. At the bottom of the plate, I pour hard agar media ( nutrient broth + 1.5% agar). Then, I mix bacteria from broth...

23 December 2023 4,941 1 View