Dear researchers,

I am new to Western blot analysis. whenever I am trying to absorb the phosphorylation of ACC (Acetyl-CoA Carboxylase) detecting by Western blot, I always observed a double band of pACC.Even several research papers observed double band but none of them explained the reason. Herewith attached the pictures of my membrane which I observed phosphorylation of ACC. Please help me to identify the reason.

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