What is the reason for bands looking uneven at the end (11-17 kda), even the protein band looks uneven after blotting
In SPSS 25, I have a categorical variable which I would like to display in a frequency table. However, one of my categories was not selected by any of my respondents. I can generate a frequency...
31 May 2024 2,076 3 View
book or article
20 May 2024 2,488 3 View
I am researching pronunciation and correct articulation among EFL students. I have a list of "problematic sounds" that students often struggle to pronounce, and I am trying to analyse those sounds...
24 December 2023 8,504 2 View
Why sodium hydroxide can cause increasing the fluorescence intensity for a fluorogenic reagent however acetone completely diminish fluorescence peak of the same reagent?
09 November 2023 3,715 1 View
What is the reason making the cyclic voltammograms looking so noisy like in the uploaded picture? is it a bad connection? something wrong with the instrument? or bad graphite source?
30 October 2023 162 3 View
Hello Scientific community, i'am seeking an oceanographer with expertise in satellite data analysis, specifically data from Sentinel-2A, to help me understand the reasons behind the excessive...
06 October 2023 8,909 0 View
I have tried some digital tools, but none of them provides a specific level/score. I need that tool for a pre-test and post-test quasi-experimental design where I implement a new program that is...
05 April 2023 6,707 3 View
What is the difference between Randles−Ševčík equation for reversible and Quasi reversible reactions during area calculation?
18 February 2023 6,601 1 View
I need your help..... I need PhD research topic suggestions in well logging analysis and interpretation. Thank you very much.
17 January 2023 412 4 View
Preparation 1-aroyl benzofuran from salisaldehyde
11 April 2022 4,866 5 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View