How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why HEK293 transfected with LAMP-1GFP do not load dextran?

Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...

14 December 2020 1,801 2 View

Why do I get weird histone bands with my Hep G2 cells??

I'm performing standard histone extraction (abcam protocol) and western blot essay on my HepG2 cells but the band's that I get don't seem to have the classic histone shape. Any clue guys?

14 December 2020 1,687 3 View

Question on ELISA logistics with modified targets?

So, I am unable to find a direct answer but essentially I want to use an ELISA protocol to measure the amount of ubiquitylated histone H2A in my mutant cells. I was wondering if I could get a kit...

02 November 2020 6,761 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View

T Cell Isolation for RT-PCR?

I need a cell that will be HLA-DR positive on RT-PCR. For this, would it work if I directly isolate RNA from PBMC cells with Ficoll? Or should I necessarily culture these PBMCs and isolate T cells...

12 October 2020 3,583 6 View

May somebody help me to standardise the amount and time of the enzyme mixture to isolate Cytotrophoblast from placenta??

i tried with the published protocols, but i am not getting any seperation in the ficol gradient. It seems like i am not incubating the 7gm minced tissue for proper time in the digestion...

03 October 2020 8,354 4 View

Why are there so many platelets in PBMC of mice isolated by Ficoll, and What should I do to dislodge these paltelets?

Hi, We used Ficoll to isolate PBMC from mice whole blood cells, but there are many little nights,with no celler structures, in PBMC through a microscope. We guess these granules are paltelets, but...

29 September 2020 1,667 5 View

How safely isolate RNA from blood of COVID-19 positive patients?

We are going to islotate RNA from patients with severe COVID-19 symptoms. How to inactivate the virus potentially present in the blood? We do not have equipment dedicated to work with viruses in...

20 September 2020 7,417 3 View

Does anybody know the difference of the effect of the dextran coated charcoal stripped serum (DCSS) containing medium and DCSS containg medium?

Hi everybody, I have cultivated the C4-2 cell line in double charcoal stripped serum containing medium. A lot of the cells do not attach to the bottom of the flask. If someone has experience...

24 August 2020 9,634 2 View

How to separate monocytes by plastic adhesion?

07 July 2020 5,883 10 View