Anthony Sabella

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Questions related from Anthony Sabella

Does TCA/acetone precipitation of histones preserve histone modifications?

I want to use isolated histones with the Abcam Histone H3 Modification Multiplex Assay Kit (ab185910) and need to know if using a trichloroacetic acid or acetone precipitation step when isolating...

17 February 2022 9,960 1 View

What is the purpose of each component of the HONDA buffer for histone extraction from plants, and are there acceptable substitutes?

Honda Buffer: 20mM HEPES-KOH pH7.4, 10mM MgCl2, 0.44M Sucrose, 1.25% Ficoll (w/v), 2.5% Dextran T40 (w/v), 0.5% Triton X-100. Before use add 0.5mM DTT, 0.5mM PMSF.

15 November 2021 9,991 0 View