Cross-linked gelatin films which are blue coloured needs to be estimated for crosslinking index by estimating the free amino groups. Do I need to use the polymeric film or polymeric film solution for estimation in case of Ninhydrin assay?
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Thank you Abdelkader BOUAZIZ . I have tried with Ninhydrin and Picrylsulfonic acid for quantiating the free amino groups. But I am not getting the absorbance linearity as the sample is blue coloured.