I want to know what is the correct protocol, thanks in advance.
Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!
10 August 2024 857 4 View
Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020? Porque requiero conocer tesis de posgrado nivel maestría...
24 July 2024 5,494 1 View
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View
Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?
14 June 2024 3,254 2 View
I am trying to do Grounded Theory in documentary research. I have obtained the most frequent terms (induction) to perform deductive coding and so, articulate a hypothesis about the hidden...
05 June 2024 2,599 2 View
I am using SU-8 2025 to generate a high aspect ratio microchannel. I made two microchannels with the properties of: 1. width = 10 um. height = 25 um. SU - 8 2025. 2. width = 15 um. height = 37.5...
27 May 2024 6,862 2 View
The yeast K. marxianus is well known for its ability to ferment de disacharide lactose. It is also able to ferment the disaccharide maltose or not? If you can share citations with respect to this...
19 May 2024 7,309 3 View
I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...
14 May 2024 2,291 2 View
14 May 2024 7,178 3 View
I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...
06 May 2024 8,974 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View