I would like to perform a standard endocytosis assay in immature and mature monocyte-derived dendritic cells in order to confirm matured status of DCs. I would like to use FluoSpheres carboxylate-modified microspheres (1um, red fluorescent), as this is the only thing available at the moment in my lab. Have you got any suggestions regarding using this method for an endocytosis assay (especially for micropinocytosis)? Successful protocols would be much appreciated!
Many thanks!