What is the process of calculating Rf factor from SDS-PAGE after protein extraction?

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Masood Sepehrimanesh

If I understand you question properly:

You must use marker for identifying the Mw of you protein in SDS PAGE

Also you can use the following image for finding more way and detail abou Rf calculation in SDS-PAGE

Vinod Kumar Jatav

markers you can use with the protein sample and run the both samples and then check the bed of protein and maker then you can easily calculate rf value