I have brain formalin fixed samples. The tissues were frozen in liquid nitrogen after 30% sucrose treatment, then sectioned with cryostat, air dried and kept in -20C. I am going to stain the tissue to observe myelin with black gold myelin staining kit. In the kit itself has mentioned that after sectioning rehydrate the tissue in milliQ water and follow the kit steps.
Now I don't know do I need to do any other fixation such as using methanol or aceton, or directly I take the sample out of the freezer, leave them in room temperature for 20 minutes and place them in milliQ water and follow the kit stpes?I truly appreciate if anyone can advice.
Hi Khadije,
I dont think that you need to fix your tissues again, since they have already been fixed, meaning that the proteins are stabilized in their location. I think you should just directly follow the directions that you have mentioned.
best wishes, Refik
Hi Khadije,
I dont think that you need to fix your tissues again, since they have already been fixed, meaning that the proteins are stabilized in their location. I think you should just directly follow the directions that you have mentioned.
best wishes, Refik
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