Shadi Khadijeh Gholami

4 Questions 18 Answers 0 Followers

Questions related from Shadi Khadijeh Gholami

What is the procedure to fix the brain frozen tissue after sectioning?

I have brain formalin fixed samples. The tissues were frozen in liquid nitrogen after 30% sucrose treatment, then sectioned with cryostat, air dried and kept in -20C. I am going to stain the...

11 November 2015 416 5 View

What is the proper quality control for very low RNA concentration prior to cDNA synthesis?

I am doing RNA extraction in which the starting material is very low and in the best situation I obtain 30 or 40 ng/ul. Since the concentration is very low it is difficult to check on agarose...

11 November 2014 4,770 6 View

Activation of dialysis sacks from sigma, to measure drug release from nanoparticle?

I need to measure the drug release from nanoparticle (the polymer is PLGA). I am going to use a dialysis sack from sigma. Do I need to activate the membrane before I use it. How do I do that? Thanks

08 August 2013 9,237 5 View

How to dissolve pva 100% hydrolyze in water.

I need to dissolve 100% hydrolyzed PVA in water. I dissolved 87% PVA in water with little heating and stirring for 2 hours. but 100% PVA I can't dissolve it even with same heating.

05 May 2013 2,370 20 View