I need to measure the drug release from nanoparticle (the polymer is PLGA). I am going to use a dialysis sack from sigma. Do I need to activate the membrane before I use it. How do I do that? Thanks
No need for activation of the membrane. check the molecule size of the drug to the pore size of the membrane. can u elaborate your work, since you mentioned PLGA?, Purpose of PLGA.
In my opinion, you have to activate membrane overnight in release medium before use it for drug release. I will send u the protocol.
If you activate membrane with the medium what your are planning to use for drug delivery study, it will help to increase accuracy and precision of drug release experiment.
Dear Khadijeh,
Here by, I am sharing the protocol.
Cut the dialysis membrane tube and immersed in boiling 1M NaHCO3 solution for about 1 hour. Then this tube is immersed in boiling RO/distilled water for 1 hr with intromitted change of water. This dialysis bag is immersed in Phosphate buffer pH 7.4 (You may use your dissolution or release medium) solution for 1 hr. Rinsed with RO/distilled water and freeze it with buffer/ release medium solution.
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