What are the limitations for colorimetric in computer vision technique to extract object features with noisy background?

Combination of morphometric and colorimetric has been applied for feature extraction in detection algorithm. In noisy background, this CV technique facing wrong identification results as well as...

07 August 2018 7,661 7 View

What is the relationship between convolution of high frequencies image with a 3 x 3 Laplacian kernel?

Filtering dataset quality is very important prior to segmentation analysis. A single channel of an image is applied before convolve it with the Laplacian kernel.

31 December 2017 9,824 0 View

What is the difference between illumination normalization techniques, ANN classifier and CNN classifier in solving face recognition problems?

This is specific to the face recognition in unconstrained environment, generalization capability of the face recognition systems with large training datasets and generation of additional synthetic...

11 December 2017 960 2 View

What is the meaning of strel, 3 x 3 matrix of all ones?

The same strel is apply for dilation and erosion operation in morphological operator.

11 December 2017 494 0 View

What is the definition and function of multiresolution segmentation in image analysis?

It was reported as a basic procedure in the software eCognition for objectoriented image analysis (Delphi2 Creative Technologies, Munich).

03 April 2017 3,254 0 View

How image intensity can be changed using the logical indexing method?

This subject is discussed under indexing in MATLAB. Basic script, Img (Img=0) = 1 (All zero intensities changed to one), or can use Img>200, depending on the aforementioned tech. used (one can...

03 April 2017 4,631 4 View

In MATLAB function, how to generate images with correlated pixels?

It is more realistic to model pixels in an images with some degree or type of correlation, because due to the point spread function and the intensity of a pixel may contain info from its...

03 April 2017 2,158 0 View

What is the relation between "Bremsstrahlung" signal and plasmons signal?

If a part of the energy that an electron carries is transferred to the specimen, several processes might occur leading to the generation of the above signals.

31 December 2016 5,573 0 View

How to determine sample size for any spectroscopic experiment?

Is there any formula or specific program?

11 December 2016 530 6 View

What is the detector for NMR spectroscopy and SEM/TEM?

To understand basic of various spectroscopy techniques, type of detector of electron with characteristic kinetic energy is important to be explained.

11 December 2016 319 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View