What is the procedure of PCR product cleaning up prior to DGGE analyses?

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Elio Fernandes

I've done DGGE and never cleaned my pcr reactions before do DGGE electrophoresis.

I agree with Ana Machado.

Other way is try improve PCR reaction, adding BSA and DMSO or changing some conditions of PCR reactions. I guess that add BSA and DMSO you can improve your reaction getting a band only.

Lucy Eland

If you have a single band in your agarose gel then there is no need to clean up your PCR products before running DGGE.