15 September 2012 1 7K Report

I am working on MS2 bacteriophage and I want to study RNA of MS2 bacteriophage.

More Nid'a Hamdan Hamad Alshraiedeh's questions See All
Forecasting with known copula parameters?

I want to calculate the Mean absolute percentage error (MAPE) for my copula model. I am stuck at the forecasting step. I am not specifying the copula here for different data pairs. 1. I have two...

14 July 2024 7,604 1 View

How to generate data from copula parameters for forecasting and model comparsion?

Hi, I have parameters of time-varying normal copula. Now I want to forecast using these parameters. For that I will need to generate data using these copulas parameters as shown in the...

20 May 2024 488 0 View

Can anyone help me identify below seaweed species?

It would be very helpful?

19 February 2024 4,633 2 View

Devise a cloning strategy to clone Chitinase gene from Spodoptera frugiperda into maize against a fungal pathogen Alternaria alternata?

If I have to make the cloning strategy, what should be my workflow to do this. I got two problems, one to select suitable expression vector from pCAMBIA1301, and pCAMBIA1302. As 1301 has GUS...

17 February 2024 8,748 2 View

For copula modeling of time series data, why are standardized residuals of fitted ARIMA/GARCH required instead of directly using time series data?

Hello, I have read that for standard copula modeling, you can get empirical cdf of data and use it for copulas. But for time series data, we must first fit ARIMA/GARCH, get standardized...

11 February 2024 2,463 1 View

About gene search in NCBI?

If the gene sequence for a specific gene (e.g., Asparaginase) is not found in the NCBI database, how can explore alternative strategies to identify and obtain the gene sequence in the organism of...

02 February 2024 1,910 3 View

Time Varying copulas Empirical analysis steps?

Hello, All papers I have read about time-varying copulas just produce tables, but none explains exact steps for empirical data. How do I calculate the time varying parameters for the copula? I...

18 December 2023 5,453 1 View

Does anyone know how to perform tuckey test and compact letter display?

I have optimized the extraction of polyphenols using BBD via RSM. I carried out the extractions in triplicates and performed TPC assay on them. Hence, for each run I have 3 extraction with 3 TPC...

18 October 2023 1,211 0 View

Obtaining natural product dry powder extract using spray drying method?

I needed some guidelines for obtaining spray dried plant extract powder. I have using 50 % aqueous ethanol as my solvent and have performed liquid extraction from my plant using shaking incubator....

05 October 2023 5,478 0 View

In alpha amylase inhibitory activity, while taking UV and, do we zero the absorbance of blank or control?

I have to conduct alpha amylase inhibitory activity but I m confused while taking readings from uv-vis spectrophotometer regarding blank and control. Do we have to zero the blank and then take...

01 October 2023 4,297 1 View

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Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

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RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

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