I need to dissolve my propolis extract (concentrated--> evaporador rotatorio) in DMSO not exceeding 0.01% in potato dextrose agar medium (200ml) and final concentration of propolis 400ug/ml in agar medium.
If this is a polar extract, like in ethanol, methanol, ethyl acetate or water, just directly dissolve in luke warm agar media- best to avoid DMSO, but if it's a hexane, chloroform like non-polar extract, then make a higher stock in 1% DMSO, or say propylene/ ethylene glycol or even some Tween 20/ Triton X (~0.1% or less) like detergent, then you can reach that final concentration. Make sure to use the same detergent, DMSO, or glycols in control plates without the propolis extract to make sure that the effect/ inhibition is not from the dissolving-agent but from the extract !
is a rotary evaporator extract of propolis , but i dont know how make a stock solution under 100% DMSO, because propolis it is not disolve
Ms Natalia, did you find the way to make a propolis stock solution.. because I think I face the same problem that the propolis not dilute in 100% DMSO. I plan to make water extraction and ethanol extraction. So, do I need to use 100% DMSO or adjust the percentage?
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