Does anyone have experience with the above test?
what is the principle behind the test?
In my new research, about TiAlN thin films, i constantly use the word "shift". I would like to know if i can write "displacement" as a synonym, with the intention to not repeat the words.
02 June 2024 9,694 4 View
We addressed such a question at Exploring The Missing Link Between Trust in Business Negotia...
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I've been trying the method described in : Reliable Exfoliation of Large-Area High-Quality Flakes of Gr... The process I'm doing now is: Clean the SiO2/Si substrates with acetone, IPA and DI...
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I tried using Fstat but the software gave me Fis and Fst for each of the loci. I am looking for Fst for each of the populations and not loci. Can anyone help me with this or suggestion useful...
30 October 2023 6,284 0 View
In the context of my PhD, I developed a Maturity Model for Responsible AI. The model uses 4 maturity levels. Each organization selects a small number of persons to fill a survey using a Likert...
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Hi everyone, I'm looking for a flow cytometry antibody that recognizes the peptide antigen (preferably OVA 323-339 or a Mtb antigen - but open to other suggestions ) bound to MHCII on mouse...
22 August 2023 795 0 View
I am currently optimizing a RPA-based protocol. As everyone knows, this nucleic acid amplification technique is based on isothermal amplification (around 37-42˚C) in combination with recombinases...
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I need to implement it so that it is not subject to human correction alone.
26 April 2023 852 3 View
Hi all, I have been trying to synthesize luciferase self-amplifying RNA (saRNA) by in vitro transcription (IVT), and this vector has approximately 11kb. The main problem is that I am getting a...
06 March 2023 611 7 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am a student in a master's program for Special Education and I am curious to see what Evidence-Based Practices that are used in teaching individuals with Autism Spectrum Disorder benefit from...
30 July 2024 5,611 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...
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if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
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I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View