Does anyone know what the preferred concentration of chitosan coated PLGA nanoparticles for MTT assay? I am starting with a 10mg/ml concentration and have not gotten good results with a 1:2 serial dilution - any suggestions?
MTT, and other colorometric cytotoxicity assays are best performed in a dose response fashion, and so a range of concentrations should be analysed. The response for a given dose will also depend on the exposure time, but typically 24 hrs is used.
As mentioned in prior answer, the range of dosage should be investigate,no one can confidently judge about that,
Also, you can search in simillar article to get insight about the aproximate range.
MTT is a colourimetric test to measure cell proliferation. Use it if you want to measure the cytotoxicity of your nanoparticles in cells.
1.- Seed cells in a 96 well polystyrene 2.- Make series of dilution of your nanoparticles an add to the cells for 24h. 3.-Add 10ul of MTT for 4h, 4.- remove the medium and then solubilized the crystals of formazan with a solvent p/e DMSO/detergent, 5.- then look for the optical density at 570
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