Hello need help on Microtome. Anyone experiencing the blade cutting deeply FFPE tissue block after a few sections of the same tissue?

The blade is set at a clearance angle of 5 degrees at which it has been sectioning very well. In the past few days with no change of blade type or type of wax. The blade has been cutting in too...

20 October 2020 769 5 View

Gromacs Protein com Ligand com distance, which is the right selection syntax for gmx distance?

I would need to check the distance between the two centers of mass of a Protein and of an organic Ligand (the resname and the resnumber of the ligand are both known), inside a system containing...

22 April 2020 3,696 4 View

How to isolate keratinocytes from fibroblasts in a co-culture using medium?

Currently I'm culturing primary human fibroblasts and keratinocytes from a skin punch in medium (DMEM with 20% FBS and 1% P/S). I would like to culture these cells separately, but I was wondering...

01 March 2020 9,425 3 View

Alternative for Qvotek microinjection needles?

A while ago the website of Qvotek went offline, and their supplies haven't been open for ordering. We were using their custom 5 micron micropipettes/needles/tips for microinjections in zebra fish...

11 November 2019 2,566 3 View

Measuring leadership Effectiveness?

I am looking for an existing tool that would assist in measuring sustainable practices on leadership effectiveness .

18 August 2018 3,640 4 View

What would be the most optimal medium for primary human VSMC culturing?

As stated in the question, I would like to know what kind of culturing medium is generally used for culturing primary human VSMC. Currently for murine cells I'm using DMEM with 10% FBS, Pen/Strep,...

10 April 2017 7,787 4 View

Thoughts about professional certification such as CISSP, CSSLP, CISA, CISM, etc. as job requirement for professors.

Do you feel that computer science and information systems programs should also require professors to have professional certifications in addition to the normal publishing requirements for tenure...

06 October 2013 1,633 6 View

Publishing in different journals vs Publishing in similar journals

For the same number of papers, is it better to publish in different journals (averaged fewer papers per journal) or publish in a few journals (averaged more papers per journal)? Does anyone have...

27 August 2012 1,223 72 View

Please can anyone refer me to a site that can give me a step-by-step procedure for artificial neural network analysis in Matlab?

01 January 1970 2,939 9 View

How can a user perception be measured for design of Virtual Learning Environments?

This question is posed in the context of designing computer simulations of real life learning scenarios using game engine platforms. Can the user experience a real life experience in the virtual...

01 January 1970 6,196 6 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

How can AI be used for enabling Design for Sustainability?

Design for Sustainability (DfS) is a wicked and complex problem. It needs the designer to combine specialized knowledge from several disciplines with systemic thinking. The designer also needs to...

05 February 2021 2,998 9 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

Can anyone help to convert ODK XLS into XML?

We want to conduct a verbal autopsy using WHO adult VA updated tools in ODK. The current version of WHO adult verbal autopsy tools is v1.5.3. I have successfully converted VA ODK-XLS v1.4 into XML...

03 February 2021 844 2 View

Does Location Map of Informal Settlements in South Africa exist?

I urgently need a map that shows the point locations of informal settlements in South Africa. I am currently working on a study that seeks to relate protests location to the location of informal...

02 February 2021 4,113 7 View

What should I do if the reviewer couldn't finalize the review in the journal's given time?

I have Submitted 2,3 Papers in Science Direct associated Journals but the time of the first decision has been ended so What should I do? Could I email them to remind them about the first decision?

01 February 2021 5,788 7 View

How the WHO reacted with covid -19in 2021?

Who is now working very hard with return of America to its active membershipnow WHO is concentrating on vaccinations,here are some activities, provides summary tables of COVID-19 vaccine...

20 January 2021 9,593 8 View

Kindly suggest sources for covid 19 data over Africa ?

Kindly suggest sources for covid 19 data for all countries of Africa included different provinces if available? Thanks in advance

17 January 2021 6,810 7 View